Antigen

Antigen Class

TFs and others

Antigen

NIPBL

Cell type

Cell type Class

Others

Cell type

Fibroblasts

NA

NA

Sample

source_name

CdLS Fibroblast

cell type

CdLS Fibroblast

antibody

NIPBL

treatment

Non-treatment

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was performed as previously described (Komata, M., et al., Chromatin immunoprecipitation protocol for mammalian cells. Methods Mol Biol, 2014. 1164: p. 33-8.). Total RNA was extracted using TRIzol (Life Technologies) and Nucleospin RNA (Macherey-Nagel) following the manufacturers' instructions. ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

50390295

Reads aligned (%)

98.2

Duplicates removed (%)

30.3

Number of peaks

13348 (qval < 1E-05)

hg19

Number of total reads

50390295

Reads aligned (%)

90.3

Duplicates removed (%)

59.7

Number of peaks

1697 (qval < 1E-05)